Product description
Also available as a customized product tailored to your needs!
OEM options:
If you need materials as in kit controls or as a third-party control for validation of your kits at customer labs, please contact us via: sales@sens-id.com
How to create your full workflow plasma control material based on SID-000128:
We recommend the use of our human DNA-free plasma products to produce complete workflow controls with this product. Blend the cfDNA as necessary with the DNA depleted plasma to receive plasma materials with the necessary DNA concentrations.
Choose your plasma volume options :
- 5 ml human Plasma (human-tech) 1-fold concentrated
- 40 ml human Plasma (human-tech) 1-fold concentrated
- 1,000 ml human Plasma (human-tech) 1-fold concentrated
This product is ideally suited for digital PCR and/or Next Generation Sequencing (NGS). Especially for
– validation and development of sequencing protocols (e.g. Amplicon sequencing) and PCR protocols
– to determine the detection limit of the method
Buffer: Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8.0
Storage: 2-8 °C
Shelf life:
Stable up to 24 months after date of manufacture (as supplied)
Quality assurance
Fragment size determination: Tapestation
Allele frequency/copy number (if indicated): dPCR
Quantification (metrologically traceable):
- dsDNA measurement (fluorometric)
Technical background
Origin: SensID’s own proprietary cell line BDXXP4
Also available on request: The Genome In A Bottle (GIAB) cell line from the Personal Genome Project (PGB): GM24385 (HG002- NA24385 – huAA53E0)
Bioinformatics:
– cell line specific sequencing files: on request
The results show BDXXP4 is a well suited wild type cell line, determined by WES (Whole Exome Sequencing).
* Limit of Blank/Limit of Detection/Limit of Quantification,
** Minimal Residual Disease