Product description
Also available as a customized product tailored to your needs!
OEM options:
If you need materials as in kit controls or as a third-party control for validation of your kits at customer labs, please contact us via: sales@sens-id.com
How to create your full workflow plasma control material based on this product:
We recommend the use of our human DNA-free plasma products to produce complete workflow controls with this product. Blend the cfDNA as necessary with the DNA depleted plasma to receive plasma materials with the necessary DNA concentrations.
Choose your plasma volume options :
- 5 ml human Plasma (human-tech) 1-fold concentrated
- 40 ml human Plasma (human-tech) 1-fold concentrated
- 1,000 ml human Plasma (human-tech) 1-fold concentrated
These products are ideal for digital PCR and/or Next Generation Sequencing (NGS). In particular:
– Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
– Determination of the detectiont limit of the method
Unit Size:
400 ng
Fragmentation size:
~167 bp
Concentration:
20 ng/µl
Mutations:
AA Change (Cosmic ID, mutation type, HGVS nomenclature, Exon)
- p.G719S (COSV51767289*, Substitution, c.2155G>A, Exon 18)
- p.E746_A750delELREA (COSV51765066*, Deletion, c.2236_2250del15, Exon 19)
- p.S752_I759delSPANKEI (COSV51774879*, Deletion, c.2254_2277del24, Exon 19)
- p.S768I (COSV51768106*, Substitution, c.2303G>T, Exon 20)
- p.V769_D770insASV (COSV51850427*, Insertion, c.2303_2304ins9, Exon 20)
- p.T790M (COSV51765492*, Substitution, c.2369C>T, Exon 20)
- p.L858R (COSV51765161*, Substitution, c.2573T>G, Exon 21)
- p.L861Q (COSV51766344 *, Substitution, c.2582T>A, Exon 21)
Allele frequency
0.1%
Buffer:
Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8.0
Storage:
2-8 °C
Expiry:
stable for 24 months from date of manufacture (as supplied)
Quality control
Fragmentation size:
High Sensitivity DNA Kit Agilent
Allele Frequency:
dPCR
Quantification:
UV-Vis Spectrophotometry (NIST-Reference method)
Qubit Fluorometry
Technical background
Derived from:
cell line GM24385 (HG002- NA24385 – huAA53E0)
Bioinformatics:
– lot specific sequencing files: LOT Search
– High-confidence variant calls: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/release/AshkenazimTrio
– Raw datasets and bam files, currently including 10X Genomics, BioNano, Complete Genomics regular and LFR, 300x Illumina paired-end, Illumina 6kb mate-pair, 1000x Ion exome, custom moleculo libraries, ~0.05x Oxford Nanopore, and 70x/30x/30x PacBio: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/data/AshkenazimTrio/HG002_NA24385_son/
*GRCh 38 Cosmic v88
