Product Description

This quality control product is ideally suited as reference material in clinical genomics workflows. In particular:

– Validation and development of extraction protocols

– control in sequencing protocols (e.g. Whole Genome Sequencing (WGS), Whole Exome Sequencing (WES), Amplicon Sequencing) and PCR protocols

– Determination of the detectiont limit of a method

– Calibration and development of instruments and workflows in DNA processing (e.g. DNA fragmentation via acoustic shearing, enzymatic digestion or sonication)

– Analyze the performance of your NGS pipeline

– routine performance monitoring (Research Use Only)

Product Overview

Format: FFPE (Formalin Fixed Paraffin Embedded)

Unit size: 1 curl per vial

Expected DNA yield: ≥ 400 ng (using Qiagen AllPrep DNA/RNA FFPE kit)

Expected RNA yield: ≥ 400 ng (using Qiagen AllPrep DNA/RNA FFPE kit)

Thickness of one curl/cut: 10 µm

Mutations:

48 cancer relevant mutations across 42 genes. List of mutated genes with specific mutations attached under “Additional Genes Covered” Tab.

Tested for PIK3CA-E542K (HGVS: c.1624G>A) with ddPCR for precise determination of allelic frequency.

The genome was sequenced and tested orthogeneously for specific mutations using ddPCR.

Allelic frequency: PIK3CA-E542K = 100%

Storage: 2-8 °C

Expiry: stable for 24 months from date of manufacturing (as supplied)

Quality control (DNA)

Allelic Frequency (metrologically traceable): ddPCR (Bio-Rad)

Quantification (metrologically traceable): Fluorometric dsDNA measurement (Qubit)

DNA Quality: Agarose gel electrophoresis

Quality Control (RNA)

RNA Quantification: Fluorometric RNA measurement (Qubit)

Quality Control (other)

Cell Density:

1. Visual via microscopy

2. Calibrated measurement

Technical background

Derived from:
human cell line

*GRCh38 COSMICv90