Product description
These products are ideal for Next Generation Sequencing (NGS) workflows. In particular:
– Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
– Calibration and development of instruments and workflows in DNA processing (e.g. DNA fragmentation via acoustic shearing, enzymatic digestion or sonication)
– Analyze the performance of your NGS pipeline by comparing to freely available datasets
Unit Size:
5 µg
Concentration:
50 ng/µl
Buffer:
Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8,0
Storage:
2-8 °C
Expiry:
stable for 24 months from date of manufacture (as supplied)
Quality control
Allelic Frequency:
Next Generation Sequencing
Quantification:
UV-Vis Spectrophotometry (NIST-reference method)
Quality:
Agarose gel electrophoresis
Technical background
Derived from:
cell line GM24385 (HG002- NA24385 – huAA53E0)
Bioinformatics:
– lot specific sequencing files: LOT Search
– High-confidence variant calls: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/release/AshkenazimTrio
– Raw datasets and bam files, currently including 10X Genomics, BioNano, Complete Genomics regular and LFR, 300x Illumina paired-end, Illumina 6kb mate-pair, 1000x Ion exome, custom moleculo libraries, ~0.05x Oxford Nanopore, and 70x/30x/30x PacBio: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/data/AshkenazimTrio/HG002_NA24385_son/
