Ashkenazim Son gDNA
"Ashkenazim Son gDNA (human)" is a highly characterized human DNA from GM24385 cell line. Details on mutation status of specific genes and Whole Genome Sequencing (WGS) data are freely available through the Personal Genome Project (PGP) and our own quality assurance.
*Not Intended for Clinical or Diagnostic Use
These products are ideal for Next Generation Sequencing (NGS) workflows. In particular:
– Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
– Calibration and development of instruments and workflows in DNA processing (e.g. DNA fragmentation via acoustic shearing, enzymatic digestion or sonication)
– Analyze the performance of your NGS pipeline by comparing to freely available datasets
Unit Size:
5 µg
Concentration:
50 ng/µl
Buffer:
Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8,0
Storage:
2-8 °C
Expiration:
stable for 24 months from date of manufacture (as supplied)
Quality control
Allelic Frequency:
Next Generation Sequencing
Quantification:
UV-Vis Spectrophotometry (NIST-reference method)
Quality:
Agarose gel electrophoresis
Technical background
Derived from:
cell line GM24385 (HG002- NA24385 – huAA53E0)
Bioinformatics:
– lot specific sequencing files: LOT Search
– High-confidence variant calls: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/release/AshkenazimTrio
– Raw datasets and bam files, currently including 10X Genomics, BioNano, Complete Genomics regular and LFR, 300x Illumina paired-end, Illumina 6kb mate-pair, 1000x Ion exome, custom moleculo libraries, ~0.05x Oxford Nanopore, and 70x/30x/30x PacBio: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/data/AshkenazimTrio/HG002_NA24385_son/
Certificate of Analysis:
Batch Certificate
other Documents:
Instructions for use
These products are ideal for Next Generation Sequencing (NGS) workflows. In particular:
– Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
– Calibration and development of instruments and workflows in DNA processing (e.g. DNA fragmentation via acoustic shearing, enzymatic digestion or sonication)
– Analyze the performance of your NGS pipeline by comparing to freely available datasets
Unit Size:
5 µg
Concentration:
50 ng/µl
Buffer:
Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8,0
Storage:
2-8 °C
Expiration:
stable for 24 months from date of manufacture (as supplied)
Quality control
Allelic Frequency:
Next Generation Sequencing
Quantification:
UV-Vis Spectrophotometry (NIST-reference method)
Quality:
Agarose gel electrophoresis
Technical background
Derived from:
cell line GM24385 (HG002- NA24385 – huAA53E0)
Bioinformatics:
– lot specific sequencing files: LOT Search
– High-confidence variant calls: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/release/AshkenazimTrio
– Raw datasets and bam files, currently including 10X Genomics, BioNano, Complete Genomics regular and LFR, 300x Illumina paired-end, Illumina 6kb mate-pair, 1000x Ion exome, custom moleculo libraries, ~0.05x Oxford Nanopore, and 70x/30x/30x PacBio: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/data/AshkenazimTrio/HG002_NA24385_son/
Certificate of Analysis:
Batch Certificate
other Documents:
Instructions for use