Product description
Recommended use:
1. DNA extraction control (e.g. instrument qualification)
2. spike-in experiments with synthetic cfDNA
3. Control in workflow validations
- Negative Control for EGFR mutations (p.G719S, p.E746_A750delELREA, p.S752_I759delSPKANKEI, p.S768I, p.V769_D770insASV, p.T790M, p.L858R, p.L861Q)
- Negative Control for 5 Gene Multiplex mutations (AKT1 – p.E17K;BRAF – p.V600E; ERBB2 – p.Y772_A775dup; KRAS – p.G12D, p.Q61K, p.A146T; PIK3CA – p.E545K, p.H1047R)
Fragment Size (Peak):
~167 bp
Unit Size:
80 ng/ml (total 400 ng)
Allelic frequencies:
100% wildtype
Buffer:
Plasma (human-tech)
Expected DNA Yield:
coming soon
Storage:
2-8 °C
Expiry:
stable for 12 months from date of manufacture (as supplied)
Quality control
Fragmentation size:
High Sensitivity DNA Kit Agilent
Allelic Frequency:
Next Generation Sequencing
Quantification:
UV-Vis Spectrometer (NIST-Reference method)
Technical background
Derived from:
cell line GM24385 (HG002- NA24385 – huAA53E0)
Bioinformatics:
– lot specific sequencing files: LOT Search
– High-confidence variant calls: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/release/AshkenazimTrio
– Raw datasets and bam files, currently including 10X Genomics, BioNano, Complete Genomics regular and LFR, 300x Illumina paired-end, Illumina 6kb mate-pair, 1000x Ion exome, custom moleculo libraries, ~0.05x Oxford Nanopore, and 70x/30x/30x PacBio: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/data/AshkenazimTrio/HG002_NA24385_son/
